Journal: BMC Microbiology

Article Title: Combatting biofilm formation of Klebsiella pneumoniae and Bacillus subtilis clinical strains from the oral cavity using biogenic Se-NPs: molecular docking simulation and cytotoxic effects on HepG2 cancer cells

doi: 10.1186/s12866-025-04142-w

Figure Lengend Snippet: Antibacterial and MIC of Se-NPs against the most virulent clinical strains. A : Hexitol as appositive control and different concentrations of Se-NPs against K. pneumoniae A11, B : Hexitol as a positive control and different concentrations of Se-NPs against B. subtilis A33 strain

Article Snippet: The National Center for Biotechnology Information (NCBI) received clinical isolates of K. pneumoniae A11 and B. subtilis A33, which were assigned accession numbers PP995146 and PP995148 , respectively.

Techniques: Control, Positive Control

Journal: BMC Microbiology

Article Title: Combatting biofilm formation of Klebsiella pneumoniae and Bacillus subtilis clinical strains from the oral cavity using biogenic Se-NPs: molecular docking simulation and cytotoxic effects on HepG2 cancer cells

doi: 10.1186/s12866-025-04142-w

Figure Lengend Snippet: Effect of Se-NPs on Biofilm activity of K. pneumoniae A11strain

Article Snippet: The National Center for Biotechnology Information (NCBI) received clinical isolates of K. pneumoniae A11 and B. subtilis A33, which were assigned accession numbers PP995146 and PP995148 , respectively.

Techniques: Activity Assay

Journal: BMC Microbiology

Article Title: Combatting biofilm formation of Klebsiella pneumoniae and Bacillus subtilis clinical strains from the oral cavity using biogenic Se-NPs: molecular docking simulation and cytotoxic effects on HepG2 cancer cells

doi: 10.1186/s12866-025-04142-w

Figure Lengend Snippet: a 3D crystallography of LuxS Synthase in K. pneumoniae (PDB: 1INN), b 3D crystallography of the biofilm matrix promoter AbbA from B. subtilis obtained from protein data bank

Article Snippet: The National Center for Biotechnology Information (NCBI) received clinical isolates of K. pneumoniae A11 and B. subtilis A33, which were assigned accession numbers PP995146 and PP995148 , respectively.

Techniques:

Journal: BMC Microbiology

Article Title: Combatting biofilm formation of Klebsiella pneumoniae and Bacillus subtilis clinical strains from the oral cavity using biogenic Se-NPs: molecular docking simulation and cytotoxic effects on HepG2 cancer cells

doi: 10.1186/s12866-025-04142-w

Figure Lengend Snippet: Structure of the Eugenol within the active site of LuxS synthase in K. pneumoniae (PDB: 1INN) ( a ) 3D Structure shown the binding mode of Se-NPs inside the pocket with amino acid interaction ( b ) 2D Structure shown the binding mode of Se-NPs inside the pocket with amino acid interaction

Article Snippet: The National Center for Biotechnology Information (NCBI) received clinical isolates of K. pneumoniae A11 and B. subtilis A33, which were assigned accession numbers PP995146 and PP995148 , respectively.

Techniques: Binding Assay

Journal: Eurosurveillance

Article Title: Cross-border spread of a mosaic resistance (OXA-48) and virulence (aerobactin) plasmid in Klebsiella pneumoniae : a European Antimicrobial Resistance Genes Surveillance Network investigation, Europe, February 2019 to October 2024

doi: 10.2807/1560-7917.ES.2025.30.27.2500439

Figure Lengend Snippet: Phylogenetic tree of Klebsiella pneumoniae isolates carrying the mosaic resistance and virulence plasmid, February 2019–October 2024 (n = 604)

Article Snippet: In addition, we completed the dataset with sequences of K. pneumoniae isolates exhibiting the key markers from previous ECDC investigations (n = 76) and from the National Center for Biotechnology Information (NCBI) Pathogen Detection and NCBI RefSeq databases (n = 1,139), as further detailed in the Supplement , excluding duplicates.

Techniques: Plasmid Preparation

Journal: Eurosurveillance

Article Title: Cross-border spread of a mosaic resistance (OXA-48) and virulence (aerobactin) plasmid in Klebsiella pneumoniae : a European Antimicrobial Resistance Genes Surveillance Network investigation, Europe, February 2019 to October 2024

doi: 10.2807/1560-7917.ES.2025.30.27.2500439

Figure Lengend Snippet: Time distribution a of Klebsiella pneumoniae isolates carrying the mosaic resistance and virulence plasmid by country, February 2019–October 2024 (n = 604)

Article Snippet: In addition, we completed the dataset with sequences of K. pneumoniae isolates exhibiting the key markers from previous ECDC investigations (n = 76) and from the National Center for Biotechnology Information (NCBI) Pathogen Detection and NCBI RefSeq databases (n = 1,139), as further detailed in the Supplement , excluding duplicates.

Techniques: Plasmid Preparation

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Molecular epidemiology of carbapenemase-producing Enterobacterales in Finland, 2012–2018

doi: 10.1007/s10096-020-03885-w

Figure Lengend Snippet: Clusters caused by carbapenemase-producing Enterobacterales in Finland, 2012–2018

Article Snippet: All clinical microbiology laboratories electronically notify E. cloacae , E. coli , and K. pneumoniae isolates with reduced susceptibility to carbapenems to the National Infectious Disease Registry and send bacterial strains with carbapenemase gene to the Expert Microbiology Unit of the THL.

Techniques: Sequencing

Journal: Pathogens

Article Title: Molecular Characterization of Presumptive Klebsiella pneumoniae Isolates from Companion and Farm Animals in Germany Reveals Novel Sequence Types

doi: 10.3390/pathogens14030259

Figure Lengend Snippet: Host, source, and MLST diversity of 53 K. pneumoniae / quasipneumoniae strains with 46 distinct STs obtained from various companion and farm animals in Germany in 2023.

Article Snippet: Sixty-four presumptive K. pneumoniae isolates from the strain collection of IDEXX Laboratories, Kornwestheim, Germany, were used in the current study.

Techniques:

Journal: Pathogens

Article Title: Molecular Characterization of Presumptive Klebsiella pneumoniae Isolates from Companion and Farm Animals in Germany Reveals Novel Sequence Types

doi: 10.3390/pathogens14030259

Figure Lengend Snippet: The phylogenetic tree of 64 K. pneumoniae / quasipneumoniae isolates from companion and farm animals in Germany was constructed using the Neighbor-Joining (NJ) method based on MLST data. The tree includes STs and corresponding geographical locations. Novel STs are indicated in red.

Article Snippet: Sixty-four presumptive K. pneumoniae isolates from the strain collection of IDEXX Laboratories, Kornwestheim, Germany, were used in the current study.

Techniques: Construct

Journal: Pathogens

Article Title: Molecular Characterization of Presumptive Klebsiella pneumoniae Isolates from Companion and Farm Animals in Germany Reveals Novel Sequence Types

doi: 10.3390/pathogens14030259

Figure Lengend Snippet: MLST characteristics of the eleven K. pneumoniae / quasipneumoniae isolates with novel STs obtained from companion and farm animals in Germany in 2023.

Article Snippet: Sixty-four presumptive K. pneumoniae isolates from the strain collection of IDEXX Laboratories, Kornwestheim, Germany, were used in the current study.

Techniques:

Journal: Microbial Genomics

Article Title: A panel of diverse Klebsiella pneumoniae clinical isolates for research and development

doi: 10.1099/mgen.0.000967

Figure Lengend Snippet: Genomic diversity of K. pneumoniae in the MRSN collection. ( a ) cgMLST minimum spanning tree of the 3123 K. pneumoniae genomes. Isolates with an identical MLST profile are represented within a single circle. The initial subset of isolates selected are indicated by filled red circles ( n =346) and the final panel isolates are indicated by filled purple circles ( n =100). ( b ) Core-genome SNP phylogenetic tree of 346 K. pneumoniae isolates initially selected to represent the breadth of K. pneumoniae diversity. The final 100 isolates selected for the panel are indicated with purple triangles. ( c ) Heatmap constructed using the format used in Fig. 6c from the manuscript by Lam et. al. showing the combination of virulence/resistance scores for all panel isolates as determined by the Kleborate typing tool. The number of isolates with a specific score is indicated in the boxes. Convergent isolates (carrying the iuc locus and an ESBL and/or carbapenemase gene) are indicated by the dashed black box and listed in the table below.

Article Snippet: The 100 K . pneumoniae panel isolates described in this study are available at BEI resources ( https://www.beiresources.org/ ) at no cost under catalogue #NR-55604.

Techniques: Construct

Journal: Microbial Genomics

Article Title: A panel of diverse Klebsiella pneumoniae clinical isolates for research and development

doi: 10.1099/mgen.0.000967

Figure Lengend Snippet: Characteristics of the K. pneumoniae diversity panel. Core-genome SNP-based phylogenetic tree of the 100 genomes in the final diversity panel. Sequence type (ST), virulence score (see legend), capsule polysaccharide locus and KL type are indicated in the columns. AMR status colours are indicated in the legend. Briefly, susceptibility results were used to classify the isolates as PDR (non-susceptible to all antibiotics tested), XDR (non-susceptible to ≥1 agent in all but ≤2 families), MDR (non-susceptible to ≥1 agent in ≥3 antibiotic families) and non-MDR (non-susceptible to one or two categories) using a modification of the criteria defined by Magiorakos et al. . The assigned antimicrobial resistance phenotype for each antibiotic tested is indicated by the maroon squares: non-susceptible (filled) or susceptible (open). The light blue circles indicate the presence of a known antimicrobial resistance gene, and the orange circles indicate the presence of a known mutation/truncation. AMK, amikacin; GEN, gentamicin; TOB, tobramycin; ATM, aztreonam; SAM, ampicillin/sulbactam; CAZ, ceftazidime; CRO, ceftriaxone; FEP, cefepime; CZA, ceftazidime/avibactam; C/T ceftolozane/tazobactam; IPM, imipenem; MEM meropenem; CIP, ciprofloxacin; LVX, levofloxacin; TZP, piperacillin/tazobactam; SXT, sulfamethoxazole–trimethoprim; TET, tetracycline; TGC, tigecycline.

Article Snippet: The 100 K . pneumoniae panel isolates described in this study are available at BEI resources ( https://www.beiresources.org/ ) at no cost under catalogue #NR-55604.

Techniques: Sequencing, Modification, Mutagenesis